The two-volume set constitutes the proceedings of the Second International Conference on Artificial Intelligence in Healthcare, AIiH 2025, which took place in Cambridge, UK, in September 2025. The 60 full papers included in this book were carefully reviewed and selected from 83 submissions. They were organized in topical sections as follows: Health informatics, Personalised Healthcare, Robotics, Assisted Living Technology, Computational Medicine, Long-term Health Conditions, Maternity and Women’s Health and Wellbeing.