This two volume set LNCS 9827 and LNCS 9828 constitutes the refereed proceedings of the 27th International Conference on Database and Expert Systems Applications, DEXA 2016, held in Porto, Portugal, September 2016. The 39 revised full papers presented together with 29 short papers were carefully reviewed and selected from 137 submissions. The papers discuss a range of topics including: Temporal, Spatial, and High Dimensional Databases; Data Mining; Authenticity, Privacy, Security, and Trust; Data Clustering; Distributed and Big Data Processing; Decision Support Systems, and Learning; Data Streams; Data Integration, and Interoperability; Semantic Web, and Data Semantics; Social Networks, and Network Analysis; Linked Data; Data Analysis; NoSQL, NewSQL; Multimedia Data; Personal Information Management; Semantic Web and Ontologies; Database and Information System Architectures; Query Answering and Optimization; Information Retrieval, and Keyword Search; Data Modelling, and Uncertainty.