This book constitutes the refereed proceedings of the 16th International Conference on Software Engineering and Formal Methods, SEFM 2018, held as part of STAF 2018, in Toulouse, France, in June 2018. The 17 full papers presented in this book were carefully reviewed and selected from 58 submissions. The papers deal with a large range of topics in the following research areas: specification; concurrency; program analysis; model checking and runtime verification; applications; and shape analysis and reuse.