This book constitutes the refereed proceedings of the 13th International Conference on Computational Advances in Bio and Medical Sciences, ICCABS 2025, held in Atlanta, GA, USA, during January 12–14, 2025. The 26 full papers presented in these proceedings were carefully reviewed and selected from 75 submissions. ICCABS has the goal of bringing together researchers, scientists, and students from academia, laboratories, and industry to discuss recent advances on computational techniques and applications in the areas of biology, medicine, and drug discovery.