Author Description

An Introduction to Biotechnology: A Genetic Manipulation Perspective contains practical exercises, and has a comprehensive coverage on the subjects of biotechnology and gene manipulation for the students' understanding of the basic concepts. This manual contains, in particular, the techniques, protocols and practical approaches that are being used routinely in molecular biology laboratories. This book is assembled together with an aim to enlighten the readers with the complex molecular biology protocols in a simple and straightforward manner. In addition, this manual also introduces readers to the study of gene manipulation in Gram-negative as well as Gram-positive bacteria. All these methods and protocols have been regularly used in the editor's laboratory and have been modified from time to time. This practical handbook overcomes the limitations of other manuals by providing an in-depth methodology in a systematic way, blending the underlying theoretical aspects, thus facilitating the readers in understanding the reason behind every step followed during the experimental work. Salient Features: Is a unique combination of protocols that are specially designed for undergraduate and postgraduate students and for those who are perusing research and teaching in Biotechnology, Molecular Biology, Botany, Zoology, Life Sciences and Microbiology. Contains primary protocols for nucleic acid isolation, protein isolation, restriction digestion, ligation, cloning, transformation, Southern hybridization and sequencing. Every chapter is well-structured and is divided into various sections: Introduction, Learning objectives, Requirements, Theoretical aspects, Procedure, Results, Discussion, Frequently asked questions and References.