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The application of molecular approaches to the identification of microorganisms indicated a substantially greater diversity of species in the periodontal environment than was previously recognized.Most common pathogenic microorganisms causing periodontitis are actinobacillus actinomy-cetemcomitans, porphyromonas gingivalis, Treponema denticola, Bacteroid, forsthus178.Pathogenic organism produces disease through a set of properties which are called as virulence factors. The application of molecular approaches to the identification of microorganisms indicated a substantially greater diversity of species in the periodontal environment than was previously recognized. Most common pathogenic microorganisms causing periodontitis are actinobacillus actinomy-cetemcomitans, porphyromonas gingivalis, Treponema denticola, Bacteroid, forsthus178.Pathogenic organism produces disease through a set of properties which are called as virulence factors.