Now in a new edition, this book provides an up-to-date account of the field of enzymology. it describes the action of enzymes in systems of increasing complexity, and shows how these properties can be best utilized, both clinically and technically. The reader will find in-depth coverage purified enzymes, including full treatment of purification methods, structure, kinetics, mechanism of action, and the control of enzyme activity. The behavior of enzymes in more complex systems is discussed, and the intact cell is thoroughy examined. Fundamentals of Enzymology also provides complete information on enzyme turnover in vivo, clinical aspects of enzymes, and enzyme technology. This new edition pays close attention to new applications of recombinant-DNA technology, and includes the latest information on the production of enzymes, the determination of amin-acid sequences, and the analysis of enzyme structure and function using site-directed mutagenesis.