"In Crystallographic Methods and Protocols leading specialists in the application of X-ray crystallography to biological problems provide a basic and comprehensive guide to its successful use in obtaining detailed three-dimensional structures of proteins, nucleic acids, and their complexes. These deeply experienced researches describe in clear practical terms their proven methods for preparing samples, crystallizing and characterizing them, acquiring and analyzing data, and solving and refining structures. They then share how to apply these state-of-the-art techniques to nucleic acids, proteins, viruses, and membrane proteins, as well as to elucidating the structure of such macromolecules as synthetic nucleic acid oligomers and protein-nucleic acid complexes.". "Crystallographic Methods and Protocols offers rich insights into and broad understanding of the strengths and limitations of X-ray crystallography. It gives biologically trained workers ready access to a powerful technique capable of helping them to solve many of today's biologically relevant problems."--BOOK JACKET.