This handbook is developed to give a solution for English teachers in facing some difficulties in the use of Animals of Nusantara virtual reality Android application for English learning. There are three units in this handbook consisted of comprehensive information about Animals of Nusantara Virtual Reality Application, the tutorial on downloading and using this application, and fifteen activities to implement this application in English learning context. This handbook also provides appendices of images related to the handbook and VR application at bit.ly/EnglishwithAoN which can be accessed freely.